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PURELINK RNA MINI KIT

日期: 2015-05-25
瀏覽次數: 545

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貨號:?12183020

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規格

Sample Type (General): Bacteria,?Yeast,?Blood,?Tissue,?Plant Samples,?Cells
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR),?Northern Blotting,?cDNA Library Construction,?Nuclease Protection Assays,?Next-Generation Sequencing,?Microarray Analysis,?Real-Time Quantitative PCR (qPCR)
Scale: Mini
System: PureLink?
Quantity: 10 columns
Product Size: 10 preps
Final Product: Total RNA
Yield (up to): 1000 μg
Number of Reactions: 10 Preps
Elution Volume - max: 300 μl
Elution Volume - min: 30 μl
Isolation Technology: Silica Spin Column
Starting Material (Amount): Up to 200 mg tissue,?5 x 10^6 to 1 x 10^8 cells
Shipping Condition: Room Temperature

內容及儲存

The PureLink? RNA Mini Kit comes with enough RNA lysis solution, spin cartridges, wash tubes, recovery tubes, wash buffers, and RNase-free water to perform 10 RNA purifications. All components should be stored at room temperature. Guaranteed stable for 1 year when properly stored.

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描述

The PureLink? RNA Mini Kit provides a simple, reliable, and rapid column-based method for isolating high-quality total RNA from a wide variety of sources without the need for hazardous reagents such as phenol. Obtain high-yield, high-integrity RNA from animal and plant cells and tissues as well as blood, bacteria, yeast, and liquid samples. The PureLink? RNA Mini Kit provides:

? Rapid, non-hazardous RNA purification capabilities
? Ready-to-use RNA for many downstream applications

Rapid and convenient column purification?
The PureLink? RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1,000 μg of purified RNA from a single extraction (see table). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with no genomic DNA contamination.

Simplified, nontoxic RNA purification
The PureLink? RNA Mini Kit combines nontoxic guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification. The safe and easy procedure can typically be completed in less than 20 minutes without the need for hazardous phenol/chloroform extraction, CsCl centrifugation, LiCl, or alcohol precipitation.
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